Yeast spotting assay with ERG7 deficient gil77 or gil77::CAS1 strains.

Growth of gil77 yeast strain transformed with the pYeDP60 vector or pYeDP60-NtCAS1 was tested on SGal medium supplemented or not with 20 µg/ml of ergosterol. After 5 days at 30°C, plates supplemented with ergosterol showed fully-grown colonies, whereas inductive conditions (SGal without ergosterol) allowed only limited growth of yeast expressing NtCAS, indicating however that NtCAS1 expression partially overcomes ERG7 deficiency.本研究针对转化了pYeDP60载体或pYeDP60-NtCAS1载体的gil77酵母菌株，检测其在添加或不添加20 μg/mL麦角固醇（ergosterol）的SGal培养基上的生长状况。在30℃下培养5天后，添加麦角固醇的平板上可形成充分生长的菌落；而在诱导培养条件（不含麦角固醇的SGal培养基）下，表达NtCAS的酵母仅能实现有限生长，但值得注意的是，NtCAS1的表达可部分弥补ERG7基因缺陷带来的影响。