S. aureus SH1000 vs Starvation transcriptional profiling (by RNA sequencing) in C. elegans and its dependence on HLH-30

In this project, our goal was to compare the transcriptional response of C. elegans to the Gram-positive pathogen S. aureus, and the metabolic stress imposed by starvation. To this end, we either infected C. elegans with S. aureus or starved them for 4h. We then extracted the RNA from the two samples and subsequently performed transcriptional profiling by RNA sequencing. RNA sequencing was done in wild type (N2- Bristol isolate) and hlh-30(tm1978) mutant animals.

本研究旨在比较秀丽隐杆线虫（C. elegans）对革兰氏阳性病原体金黄色葡萄球菌（S. aureus）的转录响应，与饥饿诱导的代谢胁迫。为达成这一目标，我们分别以金黄色葡萄球菌感染秀丽隐杆线虫，或对其施以4小时饥饿处理。随后，我们从两组样本中提取RNA，并通过RNA测序完成转录谱分析。本次RNA测序实验覆盖野生型（N2-布里斯托尔株系）及hlh-30(tm1978)突变体动物。