RNA sequencing of GES-1 cells co-cultured with H.pylori with high and low Trx1 expression. RNA sequencing of GES-1 cells co-cultured with H.pylori with high and low Trx1 expression

GES-1 gastric epithelial cells were used for co-culturing with H.pylori with high and low Trx1 expression, and RNA sequencing was performed on the co-cultured cells to explore transcriptome differences between the two groups, so as to further explore the pathogenic mechanism of Trx1 in H.pylori. Overall design: Collecting the 12h co-cultured cell precipitation included GES-1 cells without H.pylori infection (NC group), GES-1 cells co-cultured with H.pylori with high Trx1 expression (HP Trx1 high expression group) and GES-1 cells co-cultured with H.pylori with low Trx1 expression (HP Trx1 low expression group)

本研究以GES-1胃上皮细胞（GES-1 gastric epithelial cells）为实验模型，与高、低硫氧还蛋白1（Trx1）表达的幽门螺杆菌（H.pylori）进行共培养，随后对共培养细胞开展RNA测序，以探究两组间的转录组差异，进而深入剖析Trx1在幽门螺杆菌致病过程中的作用机制。实验整体设计如下：收集共培养12小时的细胞沉淀物，实验分组包含未感染幽门螺杆菌的GES-1细胞（NC组）、与高Trx1表达幽门螺杆菌共培养的GES-1细胞（HP Trx1高表达组），以及与低Trx1表达幽门螺杆菌共培养的GES-1细胞（HP Trx1低表达组）