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Single computed slice through a double tilt electron tomogram of a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form "onion skin" sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations.

本数据集包含转染突变型连接蛋白50（mutant connexin 50）的海拉细胞（HeLa cells）中观测到的层状结构的双倾斜电子断层扫描图（double tilt electron tomogram）单计算断层切片。该样本通过中压电子显微镜（intermediate voltage electron microscopy）成像，并采用四半胱氨酸-ReHAsH标记系统与荧光光氧化（fluorescence photooxidation）技术完成标记。该聚集物的同心双层膜层呈现出"洋葱皮"状片层结构，且沿长轴未形成闭合结构。样本内外侧的染色差异，大概率源于二氨基联苯胺（DAB）难以扩散进入这类聚集物的内部。