Differential gene expression of Candida albicans under no stress conditions or fluconazole stress

QUANT-seq approach was utilized to determine the gene expression in the transcriptome of C. albicans treated with the solvent control DMSO or the antifungal drug fluconazole (FLC). Biological triplicates of C. albicans grown in YPD medium treated with either DMSO or 3 µg/ml fluconazole for 30 minutes at 37°C were harvested and total RNA was isolated using standard procedures (hot phenol method). Overall design: Analysis of gene expession in C. albicans

本研究采用定量测序(QUANT-seq)方法，对经溶剂对照二甲基亚砜(Dimethyl sulfoxide, DMSO)或抗真菌药物氟康唑(fluconazole, FLC)处理的白色念珠菌(Candida albicans, C. albicans)转录组的基因表达水平进行测定。将在YPD培养基(YPD medium)中培养的白色念珠菌分为两组，分别经DMSO或3微克/毫升氟康唑于37℃条件下处理30分钟，每组设置3组生物学重复，随后收集菌体并采用标准流程（热酚法(hot phenol method)）提取总RNA。实验设计：白色念珠菌的基因表达分析。