Gene expression profiles of MCF7 breast cancer cells following estrogen and anti-estrogen treatment

We have previously demonstrated that endoxifen is the most important tamoxifen metabolite responsible for eliciting the anti-estrogenic effects of this drug in breast cancer cells expressing estrogen receptor-alpha. The goals of this study were to compare the gene expression profiles elicited by endoxifen to that of other anti-estrogens in MCF7 cells. We also examined the gene expression profiles elicited by various endoxifen concentrations in the presence of tamoxifen and its other primary metabolites in order to better understand the molecular contributions of endoxifen to the effects of tamoxifen. Total RNA was isolated from parental MCF7 cells following 24 hour treatments with various individual or combined ligands. All studies were conducted in replicates of 3.

我们此前已证实，恩多昔芬（endoxifen）是他莫昔芬（tamoxifen）最为关键的代谢产物，可在表达雌激素受体α（estrogen receptor-α）的乳腺癌细胞中介导该药物的抗雌激素效应。本研究的核心目标为比较恩多昔芬与其他抗雌激素药物在MCF7细胞中诱导的基因表达谱。此外，为深入阐明恩多昔芬对他莫昔芬药效的分子贡献，我们还在他莫昔芬及其其他主要代谢产物存在的条件下，检测了不同浓度恩多昔芬诱导的基因表达谱。我们对经不同单一或组合配体处理24小时的亲本MCF7细胞提取总RNA，且所有实验均设置3次生物学重复。