SPL9-modulated transcriptomes in age-dependent leaf development. Arabidopsis thaliana

In Arabidopsis thaliana, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE9 (SPL9), a plant-specific transcription factor targeted for repression by miR156, is an important regulator of age-dependent leaf development. To obtain a genome-wide understanding of SPL9-dependent gene expression, this RNA-seq experiment was performed. Transcriptomes of developing leaves of SPL9 loss- and gain-of-function were compared to their wild-type Col-0 counterparts, in the context of age-dependent leaf node increase and leaf maturation progression.

在拟南芥（Arabidopsis thaliana）中，SQUAMOSA启动子结合蛋白样9（SPL9）是一种被miR156靶向抑制的植物特异性转录因子，亦是年龄依赖型叶片发育的重要调控因子。为实现对SPL9依赖型基因表达的全基因组层面解析，本研究开展了本次RNA测序（RNA-seq）实验。实验以随年龄增长叶位升高、叶片成熟进程推进为背景，将SPL9功能缺失与功能获得型突变体的发育叶片转录组，与其野生型Col-0对照的转录组进行了对比分析。