Transcriptome analysis of 'Candidatus Liberibacter solanacearum' in its psyllid vector, Bactericera cockerelli [sexed insects]. Candidatus Liberibacter solanacearum

We sequenced mRNA from the bacterial pathogen 'Candidatus Liberibacter solanacearum" during its association with the psyllid vector Bactericera cockerelli. Overall design: Total RNA was purified from psyllids, insect and bacterial rRNAs were depleted. PolyA RNA was purified using Dynabeads. PolyA purified RNA and depleted RNA were sequenced.

本研究对细菌性病原体"候选韧皮部杆菌属番茄致病株（Candidatus Liberibacter solanacearum）"在与木虱传毒媒介科克罗夫特木虱（Bactericera cockerelli）共生期间的信使RNA（messenger RNA，mRNA）进行了测序。实验设计概述：从科克罗夫特木虱样本中提取总RNA，去除其中的昆虫与细菌核糖体RNA（rRNA）；采用Dynabeads磁珠纯化聚腺苷酸RNA（PolyA RNA）；随后将纯化得到的PolyA RNA与去除核糖体RNA的样本均开展测序。