Expression data from radiation-induced pulmonary fibrosis mouse models

Radiation-induced pulmonary fibrosis (RIPF) is one of the most common side effects of lung cancer radiotherapy. In the mouse lungs developing RIPF, excessive accumulation of extracellular matrix and myofibroblasts with scar formation occurs. We used microarrays to detail the global program of gene expression underlying development of radiation-induced pulmonary fibrosis and identified a variety of genes whose expression were up-regulated during this process. Seven-week-old male C57BL/6 mice were exposed to thoracic irradiation. After 25 weeks, animals were euthanized and lung samples were harvested. Development of radiation-induced pulmonary fibrosis was confirmed by western blotting for α-smooth muscle actin and hematoxylin and eosin staining.

辐射诱导肺纤维化（Radiation-induced pulmonary fibrosis, RIPF）是肺癌放射治疗最常见的不良反应之一。在发生RIPF的小鼠肺部中，会出现细胞外基质与肌成纤维细胞的过度积累，并伴随瘢痕形成。本研究采用基因微阵列（microarrays）技术，详细解析了辐射诱导肺纤维化发生发展过程背后的基因表达全局调控程序，并鉴定出该进程中表达上调的多种基因。实验选取7周龄雄性C57BL/6小鼠，对其实施胸部照射。造模25周后，对实验动物实施安乐处死并采集肺组织样本。通过针对α-平滑肌肌动蛋白（α-smooth muscle actin）的蛋白质印迹（Western blotting）实验以及苏木精-伊红染色（hematoxylin and eosin staining），确认了辐射诱导肺纤维化的造模成功。