The expression profile of miRNA in the testis of STZ-induced diabetic rats

The number of patients with diabetes is increasing worldwide. Diabetic testicular damage can cause spermiogenesis disorders and sexual dysfunction. We thus explored the role of miRNAs in diabetic testicular damage, and revealed that they could serve as effective prevention and treatment therapeutic targets.Streptozotocin (STZ) was used to generate a rat model of type 2 diabetes. Rat testicular tissues were used for miRNA sequencing. we identified 19 differentially expressed miRNAs in the testes of diabetic rats. Overall design: Testicular tissues miRNA profiles of rat after 8 weeks of STZ-induced diabetes were generated by high throughput sequencing using Illumina Hiseq 3000.

全球糖尿病患者人数逐年递增。糖尿病性睾丸损伤可引发精子生成障碍与性功能障碍。为此，本研究探讨了微小核糖核酸（miRNAs）在糖尿病性睾丸损伤中的作用，并揭示其可作为有效的防治靶点。本研究采用链脲佐菌素（Streptozotocin, STZ）构建2型糖尿病大鼠模型，提取大鼠睾丸组织进行miRNA测序，最终在糖尿病大鼠睾丸组织中鉴定出19个差异表达的miRNAs。实验整体设计：采用Illumina Hiseq 3000高通量测序平台，获取链脲佐菌素诱导糖尿病8周后大鼠的睾丸组织miRNA表达谱。