RNAi expression experiments on Illumina BeadChip. Mus musculus

Mouse embryonic stem cells (mES) were depleted of Klf2, Klf4, and Klf5 by ectopic expression of shRNA. Control RNAi was performed using shRNA against luciferase. At 2 days, 4 days and 6 days post-transfection of shRNA-encoding vectors, cells are harvested for RNA isolation. Keywords: RNAi expression, Mouse ES cells Overall design: Knockdown: Klf2, Klf4, Klf5, Luciferase (Control);Time: 2 days post-transfection, 4 days post-transfection, 6 days post-transfection;Three independent experimental replicates for each experimental condition were performed.

本研究通过异位表达短发夹RNA（short hairpin RNA, shRNA），对小鼠胚胎干细胞（Mouse embryonic stem cells, mES）中的Klf2、Klf4及Klf5基因进行敲低；对照组采用靶向荧光素酶的shRNA开展RNA干扰（RNA interference, RNAi）实验。
在转染shRNA编码载体后的第2天、第4天及第6天，收集细胞用于RNA提取。
关键词：RNA干扰表达、小鼠胚胎干细胞
实验设计：敲低靶点包括Klf2、Klf4、Klf5及荧光素酶（对照组）；样本收集时间点为转染后2天、4天、6天；每个实验条件均设置3次独立生物学重复。