K562 cell lysates prefractionated by various methods

K562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in triplicate to generate 30 raw data per method.

为对比三种预分级方法的方法学特征，研究人员采用蛋白质反相色谱（Protein Reversed-Phase Chromatography，Prot-RP）、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）以及高pH肽反相色谱（Peptide Reversed-Phase Chromatography，Pept-RP）对K562细胞裂解液进行预分级分离。针对每种方法，均将起始样品预分级为10个组分；每种预分级方法均开展三次重复实验，因此单种方法可获得30组原始数据。