siRNAs 15-29 nucleotides long with 100 percent hit to Cocksfoot streak virus

Cocksfoot grass (Dactylis glomerata) collected from Wytham, Oxford, UK, was tested for Cocksfoot streak virus infection. Small RNA of the grass was extracted and converted to DNA according to Ho, T., et al. (2008) Biochem Biophys Res Commun. 368:433-7, with primers modified to contain 454 adapter nucleotide sequences. The DNA then passed quality control through Bioanalyzer and Nanodrop before sequenced by 454 Life Sciences. Keywords: siRNA One sample analyzed by 454 high-throughput sequencing technology

鸭茅草（Dactylis glomerata）采集自英国牛津郡怀瑟姆地区，本研究针对其是否感染鸭茅草线条病毒（Cocksfoot streak virus）开展检测。参照Ho T.及其合作者2008年发表于《生物化学与生物物理研究通讯（Biochem Biophys Res Commun.）》第368卷第433-437页的实验方案，提取该草的小分子RNA（small RNA）并反转录为DNA，同时对引物进行修饰，使其携带454测序接头核苷酸序列。所得DNA样品经生物分析仪（Bioanalyzer）与超微量紫外分光光度计（Nanodrop）完成质量控制后，交由454生命科学公司（454 Life Sciences）进行测序。关键词：小干扰RNA（siRNA）；采用454高通量测序技术对单一样品进行分析。