Bifidobacterium bifidum PRL2010 actively changes the expression profiles of human cell lines

We studied the global transcription profiling of human cell lines upon colonization with Bifidobacterium bifidum PRL2010 by using DNA microarrays. We decided to use HT29 monolayer as an in vitro model to investigate the molecular impact of B. bifidum PRL2010 on human intestinal transcriptome. HT29 monolayers cultivated at 15 days of post confluence were placed in contact with PRL2010 cells for a range of time spanning from 0 h, 1 h (T1), 2 h (T2) to 4 h (T4).

本研究采用DNA微阵列（DNA microarray）技术，分析了两歧双歧杆菌（Bifidobacterium bifidum）PRL2010定植后人细胞系的全局转录组谱。本研究选用HT29单层细胞作为体外模型，旨在探究两歧双歧杆菌PRL2010对人肠道转录组的分子调控作用。将汇合后培养15天的HT29单层细胞与PRL2010菌体共孵育，处理时间覆盖0 h、1 h（T1）、2 h（T2）及4 h（T4）四个时间节点。