Prefrontal cortex transcriptome of Arid1b heterozygote mutation mice age of postnatal day 10. Prefrontal cortex transcriptome of Arid1b heterozygote mutation mice age of postnatal day 10

Arid1b is a chromatin remodeler implicated in neurodevelopmental disorders. Arid1b mutant mice with haploinsufficiency (Arid1b HT) displayed persistent excitatory synaptic dysfunction from juvenile to adult stage, decreased synaptic density and transmission. Moreover, they showed autistic-like behaviors in both of early and adult stages, decreased sociability in pup USV calling and adult social interaction, and adult repetitive grooming. To investigate early transcriptomic changes in Arid1b mutant mice, RNAseq analysis of prefrontal cortex from wild-type and Arid1b mutant mice at postnatal day 10 was done. Transcriptomic changes support these electrophysiological and behavioral deficits. Arid1b HT mice at postnatal day 10 showed alterations in genes implicated in synaptic functions and ASD. Overall design: Prefrontal cortex transcriptome of Arid1b heterozygote mutation mice age of postnatal day 10

ARID1B是一种染色质重塑因子（chromatin remodeler），其与神经发育障碍密切相关。携带单倍剂量不足（haploinsufficiency）突变的Arid1b缺陷小鼠（Arid1b HT），从幼年到成年阶段均表现出持续的兴奋性突触功能异常，突触密度与突触传递效率均显著下降。此外，该模型小鼠在幼年与成年阶段均表现出类自闭症行为：幼鼠超声波发声（ultrasonic vocalization, USV）时社交倾向降低，成年小鼠社交互动能力受损，且出现成年期重复性理毛行为。为探究Arid1b缺陷小鼠的早期转录组变化，本研究对出生后第10天的野生型与Arid1b缺陷小鼠的前额叶皮层开展了RNA测序（RNA-seq）分析。转录组学结果验证了上述电生理与行为学缺陷：出生后第10天的Arid1b HT小鼠，其与突触功能及自闭症谱系障碍（Autism Spectrum Disorder, ASD）相关的基因表达发生显著改变。实验整体设计：针对出生后第10天的Arid1b杂合突变小鼠的前额叶皮层转录组分析