SH1043992.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于物种界定、物种识别、学术交流，以及开展基于DNA的物种假说（Species Hypotheses，以下简称SH）的相关研究工作。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一套动态距离阈值（0.5%~3.0%），被聚类为近似物种级别的单元。所有SH均被赋予唯一且稳定的数字对象标识符（Digital Object Identifier，DOI）格式名称，其分类学与生态学注释信息均通过分布式网络第三方注释工作进行验证。每个SH都会结合其包含的全部序列的鉴定结果，尽可能关联到对应的分类单元名称及其分类层级（如门、纲、目等）。将为每个SH选取一条自动或手动指定的序列作为其代表序列。这些代表序列已发布至https://unite.ut.ee/repository.php，供科研社群用于本地序列相似性检索、下一代测序分析流程等各类研究场景。随着公共真菌ITS序列的数量持续增长，该系统与相关数据集将自动完成更新。