Statistical modeling, estimation, and remediation of sample index hopping in multiplexed droplet-based single-cell RNA-seq data.

Validation data for inferring the sample index hopping rate. The hiseq4000_joined_datatable_plexed_nonplexed.zip file contains read counts for four samples (two non-multiplexed and two multiplexed)  joined by  a cell-barcode, UMI, and gene-ID (CUG) key combination. The hiseq4000_inner_joined_with_labels.zip file contains only those CUGs that are observed in both the non-multiplexed and multiplexed samples.

本数据集为用于推断样本索引跳变率的验证数据。其中，hiseq4000_joined_datatable_plexed_nonplexed.zip 文件包含以细胞条形码（cell-barcode）、唯一分子标识符（Unique Molecular Identifier，UMI）与基因ID（gene-ID）的组合密钥（CUG密钥）为拼接键的四个样本的测序读段计数数据，该四个样本涵盖2个非多重测序样本与2个多重测序样本。而hiseq4000_inner_joined_with_labels.zip 文件仅收录了同时在非多重测序样本与多重测序样本中均被观测到的CUG密钥对应的测序读段计数数据。