Single cell RNA sequencing reveals heterogeneity and dynamics of mouse early inguinal white adipose tissue (iWAT) during development. Single cell RNA sequencing reveals heterogeneity and dynamics of mouse early inguinal white adipose tissue (iWAT) during development

In this study, we comprehensively compared the changes in adipose tissue from 1 week (7 d) to 4 weeks (28 d) postnatally. To analyze the different cell groups and how their amount and function vary along with developmental time, we collected iWAT from 7-, 14-, 18-, 21-, and 28-day-old mice and enzymatically disassociated the tissues into single cells and performed single-cell RNA sequencing. Overall design: To profile cells in an unbiased manner from iWAT considering the apparent alterations in phenotype and gene expression in mouse iWAT during early life, we collected iWAT from 7-, 14-, 18-, 21-, and 28-day-old mice and enzymatically disassociated the tissues into single cells. Stromal vascular cell fractions (SVFs) were separated from mature lipid-containing adipocytes by centrifugation and were subjected to single-cell RNA-seq.

本研究全面比较了小鼠出生后1周（7日龄）至4周（28日龄）的脂肪组织变化。为解析不同细胞群及其数量与功能随发育进程的变化规律，我们采集了7、14、18、21及28日龄小鼠的腹股沟白色脂肪组织（inguinal white adipose tissue, iWAT），通过酶解法将组织解离为单个细胞，并开展了单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）。实验设计：鉴于小鼠早期发育阶段腹股沟白色脂肪组织的表型与基因表达存在显著改变，为实现无偏的细胞谱分析，我们采集了上述日龄小鼠的腹股沟白色脂肪组织，经酶解法解离为单个细胞后，通过离心将基质血管组分（stromal vascular cell fractions, SVFs）与成熟含脂脂肪细胞分离，随后对基质血管组分开展单细胞RNA测序。