EZH2 occupancy profiling by high throughput sequencing from macrophages non-treated (NT) or stimulated with LPS. Mus musculus

To identify the potential genes that regulated by Ezh2, we immunoprecipitated chromatin from macrophages stimulated with or without LPS, and analyzed the precipitated DNA with deep sequencing (CHIP-Seq). Overall design: To examine EZH2 modification in macrophages,the chromatin of macrophages non-treated (NT) or stimulated for 2 h with LPS was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against EZH2 ( Active Motif 39901).

为鉴定受Ezh2调控的潜在靶基因，我们对经脂多糖（lipopolysaccharide, LPS）刺激或未刺激的巨噬细胞开展染色质免疫沉淀（chromatin immunoprecipitation, ChIP）实验，并通过深度测序对沉淀富集得到的DNA进行分析，即ChIP测序（ChIP-Seq）。实验整体设计：为检测巨噬细胞内EZH2的修饰状态，我们分别制备未处理（NT，non-treated）组与经LPS刺激2小时组的巨噬细胞染色质样本，随后交由Active Motif公司使用靶向EZH2的特异性抗体（货号：Active Motif 39901）完成ChIP-Seq分析。