The effect of inhibition of receptor tyrosine kinase AXL on DNA damage response in ovarian cancer

To explore molecular alterations upon AXL inhibition and the functions that might be regulated by AXL and SAM68 Overall design: Next, we performed RNA-sequencing on OV cells treated with DMSO or R428, OV cells with AXL knockout and OV cells with SAM68 KO to map out common biological processes distinctive to respective cell type and to compare their gene expression profile We performed gene expression profiling analysis using data obtained from RNA-seq of 1 cell line treated with AXL inhibition at two time points, AXL knockout cell line and SAM68 knockout cell line

为探究AXL抑制后的分子改变，以及AXL与SAM68可能调控的生物学功能
实验设计：我们对经二甲基亚砜（DMSO）或R428处理的OV细胞、AXL敲除的OV细胞以及SAM68敲除的OV细胞进行RNA测序（RNA-sequencing），以厘清各对应细胞类型特有的共有生物学过程，并比较其基因表达谱。本研究基于1株细胞系的RNA测序数据开展基因表达谱分析，该细胞系经AXL抑制处理后设置了两个时间点，同时包含AXL敲除细胞系与SAM68敲除细胞系