Steady-state voltage-dependent activation and inactivation parameters of various rEag1 constructs.

Unless stated otherwise, the voltage-dependent gating property of rEag1 channels was determined from steady-state currents recorded with 3 mM external KCl. Reversal potentials (as determined from I–V curves) and steady-state current amplitudes were used to calculate channel conductances at different membrane potentials, which in turn were normalized to the maximum amplitude to obtain the relative Po. Alternatively, for rEag1-chimera P and N, isochronal tail currents (in 3 mM KCl) were normalized to the maximum amplitude to obtain corresponding relative Po values. Data points for non-inactivating and inactivating channels were fit with one and two Boltzmann functions, respectively (see Methods for more detail). Curve fitting parameters: V0.5a and V0.5i represent the half-maximal voltage for activation and inactivation, respectively; ka and ki the slope factor for activation and inactivation, respectively; and for inactivating channels only, b the non-inactivating relative Po at depolarized potentials. Data are shown as mean ± SEM (*, significantly different from rEag1-WT; t-test: pSteady-state voltage-dependent activation and inactivation parameters of various rEag1 constructs.