Transcriptome changes upon Cas13-based knockdown

We used RNA-seq to detect genome-wide gene expression change when a reporter RNA was targeted by Cas13 Examination of two gRNAs and two target RNA concentrations in HEK293T cells Transfection in HEK293T cells with RfxCas13d (CasRx with NLS), target reporter (DsRed), control reporter (BFP), and gRNA (g3: targeting, g11: control).

我们采用RNA测序（RNA-seq）技术，检测报告RNA（reporter RNA）被Cas13靶向时的全基因组基因表达变化。本研究在HEK293T细胞中设置了两种向导RNA（gRNAs）与两种靶RNA浓度组合开展实验分析。本次转染实验向HEK293T细胞中导入了以下组分：RfxCas13d（携带核定位信号的CasRx）、靶标报告基因（DsRed）、对照报告基因（BFP）以及向导RNA（其中g3为靶向型gRNA，g11为对照型gRNA）。