SH1043735.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及处理基于DNA的物种假说（DNA-based Species Hypotheses, SH）提供了统一的技术框架。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer, ITS）序列，通过应用一组动态距离阈值（0.5%至3.0%区间，含低于0.5%的取值），被聚类至近似物种水平的聚类单元。所有物种假说均被赋予以数字对象标识符（Digital Object Identifier, DOI）为格式的唯一且稳定的专属名称，其分类学与生态学注释信息需通过基于网络的分布式第三方注释流程完成验证。每个物种假说将尽可能结合其包含的全部序列的鉴定结果，与对应分类单元名称及其分类层级（如门、纲、目等）建立关联。针对每个此类物种假说，系统将自动或手动选定一条序列作为其代表序列。此类代表序列已通过https://unite.ut.ee/repository.php对外发布，可供科研社群用于本地序列相似性搜索、高通量测序分析流程等科研场景。随着公共真菌ITS序列的规模持续增长，该系统及其配套数据将自动完成更新。