Expression profiling of the wild type, mot2∆, mamRNA∆ and mot2∆ mamRNA∆ strains. Expression profiling of the wild type, mot2∆, mamRNA∆ and mot2∆ mamRNA∆ strains

Transcriptome analyses of wild type, mot2∆, mamRNA∆ and mot2∆ mamRNA∆ strains. We determined the role of mamRNA in the accumulation of Mmi1-targeted transcripts observed in mot2∆ cells. Overall design: Total RNAs from wild type (duplicate), mot2∆ (duplicate), mamRNA∆ (duplicate) and mot2∆mamRNA∆ (duplicate) strains were analyzed by high-throughput sequencing

本研究针对野生型（wild type）、mot2Δ、mamRNAΔ及mot2Δ mamRNAΔ菌株开展转录组分析，以明确mamRNA在mot2Δ细胞中观测到的Mmi1靶向转录本积累过程中的作用。实验设计概述：采用高通量测序技术，对野生型菌株（2次生物学重复）、mot2Δ菌株（2次生物学重复）、mamRNAΔ菌株（2次生物学重复）以及mot2Δ mamRNAΔ菌株（2次生物学重复）的总RNA进行测序分析。