Zone dependent distinctive gene expression profile of the normal human liver tissue. Homo sapiens

The regulatory mechanisms that shapes the hepatic zonation is not well understood. In addition, the concept and significance of of hepatic zonation is well established in rodens, however, its relavence to human liver biology remain elusive. We conducted a comprehensive transcriptome analysis of each zonation within normal human liver vis Laser Capture Microdissection approach. Here, we report a poly A RNA sequencing data of the individual zone of liver tissue as well as the whole liver of the corresponding subjects. Overall design: The RNA samples were collected from each zone within hepatic lobule by a Laser Captured Microdissection approach. This study examined the gene expression profile in each zone of the normal human liver.

调控肝脏分区（hepatic zonation）形成的分子机制目前尚未得到充分阐释。此外，尽管肝脏分区的概念及其生物学意义在啮齿类动物中已得到充分确立，但其与人类肝脏生物学的相关性仍不甚明确。本研究采用激光捕获显微切割（Laser Capture Microdissection）技术，对正常人类肝脏内的各个分区开展了全面的转录组分析，并公开了对应受试者肝脏单个分区及全肝组织的poly A RNA测序数据。
实验设计概述：本研究通过激光捕获显微切割技术，从肝小叶的各个分区中获取RNA样本，解析了正常人类肝脏各分区的基因表达谱。