Identification of target genes of miR-31 in PTC cells by RNA sequencing. Identification of target genes of miR-31 in PTC cells by RNA sequencing

The purposes of this study were to compare and analyze the expression differences of papillary thyroid carcinoma (PTC) induced by BrafV600E after knockdown of miR-31 deletion. And in order to gain insights into global gene expression regulated by miR-31 in human PTC cells, RNA-Seq experiments were performed in K1 cells with knockdout of miR-31. Overall design: mRNA profiles of K1-control and K1-miR-31 KO were generated by deep sequencing, in duplicate, using Illumina novaseq6000 .

本研究旨在对比分析BRAF V600E诱导的甲状腺乳头状癌（papillary thyroid carcinoma, PTC）在微小RNA-31（miR-31）敲除后的基因表达差异；同时为深入解析人类PTC细胞中miR-31调控的全局基因表达谱，本研究对miR-31敲除的K1细胞开展了RNA测序（RNA-Seq）实验。
总体设计：采用Illumina NovaSeq6000测序平台，对K1对照组与K1-miR-31敲除组进行两次生物学重复的深度测序，以获取其mRNA表达谱。