RNA sequencing of adipose tissue-derived stromal cells (ADSCs) with or without Sal-B addition

Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza. Studies have shown that salvianolic acid B increases the mRNA expression of adipogenic transcription factors, including PPARγ, C/EBPα and PPARα, in 3T3-L1 preadipocytes to increase glucose uptake and mitochondrial respiration, reduce glycerol release and promote adipocyte differentiation. However, the effect of Sal-B on graft fat survival and its specific mechanism have not been investigated. So we dicided using h-ADSCs to see its transcriptomes changing after Sal-B addition. Examination of h-ADSCs RNA sequencing comparing the Sal-B addition(50μmol/L, four days and eight days) group and control group

丹酚酸B（Salvianolic acid B, Sal-B）是丹参（Salvia miltiorrhiza）中含量最高且活性最强的水溶性活性成分。已有研究表明，丹酚酸B可在3T3-L1前脂肪细胞中上调脂肪生成相关转录因子（包括过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT增强子结合蛋白α（C/EBPα）与过氧化物酶体增殖物激活受体α（PPARα））的mRNA表达水平，从而促进葡萄糖摄取与线粒体呼吸、降低甘油释放并加速脂肪细胞分化。然而，丹酚酸B对移植脂肪存活的影响及其具体作用机制尚未得到阐明。为此，本研究选用人类脂肪来源干细胞（human adipose-derived stem cells, h-ADSCs），以探究丹酚酸B处理后其转录组的变化情况。本研究通过RNA测序比较丹酚酸B处理组（50μmol/L，分别处理4天与8天）与对照组的样本差异。