Comparative analysis of D. shibae wild-type vs. delta-luxI1 supplemented with different acetylated homoserine-lactones

Comparison of a quorum sensing null mutant supplemented with C18-HSL, C18en-HSL, C18dien-HSL with D. shibae wild-type in order to identify traits induced by distinct autoinducers in this organism. Loop-design, two to three biological replicates of D. shibae wild-type, delta-luxI1 and delta-luxI1 supplemented with 500 nM C18-HSL, C18en-HSL and C18dien-HSL at cultivation start. Samples were taken in mid-exponential growth phase at OD600 0.4

本研究旨在鉴定希巴赤杆菌（Dinoroseobacter shibae，D. shibae）中不同自体诱导物（autoinducer）所诱导的生物学性状，为此对补充了C18-HSL、C18en-HSL、C18dien-HSL的群体感应（quorum sensing）缺失突变株与该菌株野生型进行比较分析。实验采用循环设计（Loop-design），设置2-3次生物学重复，实验组涵盖D. shibae野生型、ΔluxI1缺失株，以及在培养起始时添加500 nM C18-HSL、C18en-HSL、C18dien-HSL的ΔluxI1缺失株。于指数生长中期（OD600值为0.4）采集实验样本。