Table1_Characterization and gene expression patterns analysis implies BSK family genes respond to salinity stress in cotton.xlsx

Identification, evolution, and expression patterns of BSK (BR signaling kinase) family genes revealed that BSKs participated in the response of cotton to abiotic stress and maintained the growth of cotton in extreme environment. The steroidal hormone brassinosteroids (BR) play important roles in different plant biological processes. This study focused on BSK which were downstream regulatory element of BR, in order to help to decipher the functions of BSKs genes from cotton on growth development and responses to abiotic stresses and lean the evolutionary relationship of cotton BSKs. BSKs are a class of plant-specific receptor-like cytoplasmic kinases involved in BR signal transduction. In this study, bioinformatics methods were used to identify the cotton BSKs gene family at the cotton genome level, and the gene structure, promoter elements, protein structure and properties, gene expression patterns and candidate interacting proteins were analyzed. In the present study, a total of 152 BSKs were identified by a genome-wide search in four cotton species and other 11 plant species, and phylogenetic analysis revealed three evolutionary clades. It was identified that BSKs contain typical PKc and TPR domains, the N-terminus is composed of extended chains and helical structures. Cotton BSKs genes show different expression patterns in different tissues and organs. The gene promoter contains numerous cis-acting elements induced by hormones and abiotic stress, the hormone ABA and Cold-inducing related elements have the highest count, indicating that cotton BSK genes may be regulated by various hormones at different growth stages and involved in the response regulation of cotton to various stresses. The expression analysis of BSKs in cotton showed that the expression levels of GhBSK06, GhBSK10, GhBSK21 and GhBSK24 were significantly increased with salt-inducing. This study is helpful to analyze the function of cotton BSKs genes in growth and development and in response to stress.

本研究通过对BR信号激酶（BR signaling kinase，BSK）家族基因的鉴定、进化分析及表达模式探究，发现BSK家族成员参与棉花对非生物胁迫的应答过程，且可维持棉花在极端环境下的生长。类固醇激素油菜素内酯（brassinosteroids，BR）在多种植物生物学过程中发挥关键调控作用。本研究以BR的下游调控因子BSK为对象，旨在解析棉花BSK家族基因在生长发育与非生物胁迫应答中的功能，并明确棉花BSK家族的进化关系。BSK是一类参与BR信号转导的植物特异性类受体胞质激酶。本研究采用生物信息学方法，在棉花全基因组水平上鉴定BSK家族基因，并对其基因结构、启动子顺式作用元件、蛋白质结构与特性、基因表达模式以及候选互作蛋白进行了系统分析。本研究通过全基因组检索，在4个棉花物种及另外11种植物中共鉴定得到152个BSK家族基因，系统发育分析显示其可划分为3个进化支。研究发现，BSK家族蛋白含有典型的PKc结构域与TPR结构域，其N端由延伸链与螺旋结构共同构成。棉花BSK家族基因在不同组织器官中呈现特异性表达模式。其基因启动子区域含有大量激素与非生物胁迫诱导的顺式作用元件，其中脱落酸（ABA）响应元件与低温诱导相关元件的数量最多，表明棉花BSK基因可在不同生长阶段受多种激素调控，并参与棉花对多种胁迫的应答调控。棉花BSK基因的表达分析结果显示，GhBSK06、GhBSK10、GhBSK21与GhBSK24的表达水平在盐胁迫诱导后显著上调。本研究可为解析棉花BSK家族基因在生长发育及胁迫应答中的功能提供理论参考。