Equine OA model - An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro

Articular cartilage explants were incubated with or without interleukin-1β for 25 days. Media samples collected at 3-day intervals were analysed by quantitative proteomics. Semi-tryptic COMP peptides containing novel neo-epitopes were identified and quantified in cell culture media from cartilage explants.

关节软骨外植体（articular cartilage explants）分别在添加与不添加白细胞介素-1β（interleukin-1β）的培养基中孵育25天。每3天采集一次培养基样本，通过定量蛋白质组学（quantitative proteomics）技术进行分析。最终在软骨外植体来源的细胞培养液中，鉴定并定量得到了含有新型新表位（neo-epitopes）的半胰蛋白酶COMP肽段（semi-tryptic COMP peptides）。