Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly

With advances in high-throughput sequencing technologies, de novo transcriptome sequencing and assembly has become a cost-effective method to obtain comprehensive genetic information of a species of interest, especially in non-model species with large genomes such as spiders. However, high-quality RNA is essential for successful sequencing and sample preservation conditions require careful consideration for the effective storage of field-collected samples. To this end, we report a streamlined feasibility study of various storage conditions and their effects on de novo transcriptome assembly results. The storage parameters considered include temperatures ranging from room temperature to -80°C; preservatives, including ethanol, RNAlater, TRIzol, and RNAlater-ICE; and sample submersion states. As a result, intact RNA was extracted and assembly was successful when samples were preserved at low temperatures regardless of the type of preservative used. The assemblies as well as the gene expre...

随着高通量测序技术的进步，从头转录组测序与组装（de novo transcriptome sequencing and assembly）已成为获取目标物种全面遗传信息的高性价比手段，尤其适用于蜘蛛这类拥有大基因组的非模式物种。然而，高质量RNA是测序成功的必要前提，野外采集样本的有效保存需谨慎规划保存条件。为此，本研究针对多种保存条件及其对从头转录组组装结果的影响开展了精简可行性研究。本次研究所考量的保存参数包括：室温至-80℃的温度区间、乙醇、RNAlater、TRIzol、RNAlater-ICE等保存剂，以及样本浸没状态。结果表明，无论使用何种类型的保存剂，仅在低温条件下保存的样本均可提取得到完整RNA并成功完成组装。本次组装结果以及基因表...