Transcriptome profiling of inflammatory monocytes and macrophages in breast cancer bone metastasis. Transcriptome profiling of inflammatory monocytes and macrophages in breast cancer bone metastasis

Macrophages play a critical role in promoting bone metastasis growth. Microarray was used for determining the transcriptional change of macrophages and their progenitor inflammatory monocytes during bone metastasis progression. Overall design: We sorted bone marrow macrophages (Macs) and Ly6C+ inflammatory monocytes (IMs) from mice bearing MetBo2 bone metastasis and from healthy bone marrow. Labeling and hybridization were done in Einstein Microarray Facility using Affymetrix MoGene 2.0 st chips. A total of 19 samples (10 healthy and 9 bone metastasis, 4-5 replicates for each cell type) was profiled.

巨噬细胞在促进骨转移瘤生长过程中发挥关键作用。
本研究采用基因芯片（Microarray）技术，检测骨转移进展过程中巨噬细胞及其前体炎性单核细胞的转录表达变化。
实验设计概述：我们从携带MetBo2骨转移的小鼠与健康小鼠的骨髓中，分选得到骨髓巨噬细胞（Macs）与Ly6C+炎性单核细胞（IMs）。
样本标记与杂交实验均在爱因斯坦基因芯片平台（Einstein Microarray Facility）完成，所用芯片为Affymetrix MoGene 2.0 ST基因芯片。
本研究共对19份样本进行表达谱分析，其中健康对照组10份、骨转移模型组9份，每种细胞类型设置4至5个生物学重复。