Miz-1 is required to coordinate the pre-TCR-dependent transitional step in DN3/DN4 thymocytes. Mus musculus

Lineage negative, CD44 negative, CD25 positive thymocytes were isolated from wt mice or Miz1 POZ-domain knockout mice to analyze the effect of loss of Miz1 in the DN3 population of T-cells We used the mouse Affymetrix MOE430-2 microarray to characterize global gene expression changes of DN3 thymocytes from Miz1 knockout mice. Overall design: We performed microarray based gene expression profiling to determine the effect of loss of Miz1 activity on the gene expression pattern of mouse DN3 thymocytes from wt and Miz1-delta-POZ mice.

我们从野生型（wild type, wt）小鼠及Miz1 POZ结构域敲除（Miz1 POZ-domain knockout）小鼠中分离得到谱系阴性（Lineage negative）、CD44阴性、CD25阳性的胸腺细胞，以此探究Miz1缺失对T细胞DN3亚群的影响。为表征Miz1敲除小鼠DN3胸腺细胞的全局基因表达变化，我们使用小鼠Affymetrix MOE430-2基因芯片开展实验。
实验设计：本研究通过基于基因芯片的基因表达谱分析，明确Miz1活性缺失对野生型（wt）及Miz1-ΔPOZ小鼠的DN3胸腺细胞基因表达模式的影响。