Proteomic analysis of macrophages derived from induced pluripotent stem cells

Macrophages (Mɸ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated Mɸ can exist in two extreme phenotypes: pro-inflammatory (M1) and anti-inflammatory (M2) Mɸ and these phenotypes can be recapitulated in vitro by using lipopolysaccharide (LPS) plus IFNγ and IL-4, respectively. In the recent years, human induced pluripotent stem cells (iPSC) derived-Mɸ have gained major attention since they are functionally similar to human monocyte derived-Mɸ and are receptive to genome editing. In this study, we used quantitative proteomics to address whether the plasticity of iPSC-derived Mɸ (iPSDM) are similar to human monocyte derived Mɸ. Our analyses suggest that iPSC Mɸ are promising tools to understand Mɸ biology since they exhibit similar polarisation profiles and functions as monocyte-derived Mɸ. We believe this comprehensive proteome data set will be a useful resource in the Mɸ field.

巨噬细胞（Macrophages，缩写Mɸ）是一类高度异质性且功能多样的固有免疫细胞，参与机体稳态维持与免疫应答。活化的巨噬细胞可分为两种极端表型：促炎型（M1）与抗炎型（M2）巨噬细胞，二者在体外可分别通过脂多糖（LPS）联合干扰素γ（IFNγ）、白细胞介素4（IL-4）诱导获得。近年来，人类诱导多能干细胞（iPSC）来源的巨噬细胞获得了广泛关注，因其功能与人类单核细胞来源的巨噬细胞相似，且易于进行基因组编辑。本研究采用定量蛋白质组学技术，探究人类诱导多能干细胞诱导分化的巨噬细胞（iPSDM）的可塑性是否与单核细胞来源的巨噬细胞一致。分析结果表明，iPSC来源巨噬细胞展现出与单核细胞来源巨噬细胞相似的极化谱与功能，是研究巨噬细胞生物学的极具潜力的工具。我们认为，本研究产生的全面蛋白质组数据集将为巨噬细胞领域的相关研究提供宝贵的资源。