Development of Diversity Arrays Technology markers as a tool for rapid genomic assessment in Nile tilapia, Oreochromis niloticus

The development of genomic markers is described for Nile tilapia, Oreochromis niloticus, using the Diversity Arrays Technology (DArT) genotype-by-sequencing platform. A total of 13 215 single nucleotide polymorphism (SNP) markers and 12 490 silicoDArT (dominant) markers were identified from broodstock of two selective breeding programs [Genetically Improved Farmed Tilapia (GIFT) strain from Malaysia and the Abbassa strain from Egypt]. Over 10 000 SNPs were polymorphic in either strain, and 2985 and 3087 showed strain-specific polymorphisms for the GIFT and Abbassa strains respectively. We demonstrate the potential utility of these markers for rapid genomic screening and use in breeding programs.

本研究针对尼罗罗非鱼（Oreochromis niloticus），基于多样性阵列技术（DArT）分型测序平台开展基因组标记开发。从两项选育计划的亲本群体中，共鉴定得到13215个单核苷酸多态性（SNP）标记与12490个硅基DArT显性标记；这两项选育计划的亲本分别源自马来西亚的吉富（GIFT）品系与埃及的阿巴萨品系。两个品系中均有超过10000个SNP标记呈现多态性，其中吉富品系与阿巴萨品系分别包含2985个和3087个品系特异性多态SNP标记。本研究证实了上述标记可用于快速基因组筛选，并可应用于育种项目中。