raw data for quantification of D12 and D18/19 immunocytochemistry
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Underlying data for quantification graphs shown in Figure 3<br>cppipe files: CellProfiler v.3.1.9 was used to generate the pipelines for each image set (one differentiation protocol, 3 channels, 2 timepoints).<br>quantification.xlsx: The raw data generated after running the pipelines were copied to a spreadsheet. 'Cells / Nuclei (%)' column shows the percentage of cells positive for SOX2 at D12 and TBR2 at D18/19. 'Intracellular APOE / Cells (%)' column shows the percentage of intracellular APOE-positive cells amongst SOX2- and TBR2-positive cells. These 2 columns are presented as bar graphs on Figure 3 of the manuscript.<br>
图3所示定量图表的基础数据集<br>cppipe文件:使用CellProfiler v3.1.9为每组图像集生成分析管道(涵盖1种分化方案、3个通道、2个时间点)。<br>quantification.xlsx:运行上述分析管道后得到的原始数据被导入电子表格。其中,"细胞/细胞核占比(%)"列展示了D12时点SOX2阳性细胞、D18/19时点TBR2阳性细胞的占比;"胞内APOE/细胞占比(%)"列则展示了SOX2与TBR2阳性细胞中,胞内APOE阳性细胞的占比。上述两列数据以柱状图形式呈现于本文稿的图3中。
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figshare
创建时间:
2020-08-08



