Open-source sensor for measuring oxygen partial pressures below 100 microbars

The ability to measure partial pressures of oxygen below 100 microbars and nanomolar dissolved oxygen concentrations in in situ laboratory systems benefits many fields including microbiology, geobiology, oceanography, chemistry, and materials science. Here, we present an easily constructible open-source design for a networked luminescence lifetime measurement system for in situ measurements in arbitrary laboratory containers. The system is well suited for measuring oxygen partial pressures in the 0–100 μbar range, with the maximum potentially usable upper range limit at around 10 mbar, depending on experimental conditions. The sensor has a limited drift and its detectability limit for oxygen is at 0.02 μbar for short timescale measurements. Each sensor can connect to a Wi-Fi network and send the logged data either over the Internet or to a local server, enabling a large number of parallel unattended experiments. Designs are also provided for attaching the sensor to various commercially available containers used in laboratories. The design files are released under an open source license, which enables other laboratories to build, customize, and use these sensors.

在原位（in situ）实验室体系中，测量100微巴（microbar）以下氧气分压与纳摩尔级溶解氧浓度的能力，可惠及微生物学、地质生物学、海洋学、化学及材料科学等诸多研究领域。
本研究提出一种易于搭建的开源设计方案，用于构建可联网的发光寿命（luminescence lifetime）测量系统，以实现对各类实验室容器内环境的原位测量。
该系统适配0~100微巴区间的氧气分压测量，其理论可用量程上限可达约10毫巴（mbar），实际上限取决于具体实验条件。
该传感器漂移极低，短期测量场景下的氧气检测限可达0.02微巴。
每个传感器均可接入Wi-Fi网络，可通过互联网或本地服务器上传已记录的实验数据，从而支持开展大规模并行的无人值守实验。
本设计还提供了将传感器适配至各类商用实验室容器的安装方案。
本研究的设计文件以开源许可证发布，可供其他实验室搭建、定制并使用此类传感器。