Effect of muscle-specific Pex5 depletion on gene expression in gastrocnemius muscle in 3 months-old mice

We generated a muscle-specific Pex5 KO mouse to investigate the consequences of peroxisomal dysfunction on muscle homeostasis. Our study reveals that peroxisomal impairment triggers early alterations in skeletal muscle lipid and amino acid metabolism and disrupts the physical interaction between peroxisomes and mitochondria, resulting in reduction of muscle force and exercise performance. Over time, these disruptions lead to metabolic changes that affect mitochondrial content, and function anticipating the development of age-related muscle dysfunction. Total RNA was extracted from n=4 control and n=5 Pex5-null gastrocnemius muscle

本研究构建了肌肉特异性Pex5基因敲除（Pex5 KO）小鼠，以探究过氧化物酶体（peroxisome）功能异常对肌肉稳态的影响。研究结果显示，过氧化物酶体功能损伤会诱发骨骼肌脂质与氨基酸代谢的早期异常，并破坏过氧化物酶体与线粒体（mitochondrion）之间的物理相互作用，最终导致肌肉力量与运动能力下降。随着时间推移，上述紊乱会引发代谢改变，影响线粒体的含量与功能，进而预示年龄相关性肌肉功能障碍的发生发展。本研究从n=4只对照组小鼠与n=5只Pex5基因敲除（Pex5-null）小鼠的腓肠肌中提取了总RNA。