Effect of HNF4α overexpression on gene expression profile. Effect of HNF4α overexpression on gene expression profile

In order to explore the potential mechanism of HNF4α in ICC, RNA-seq was performed to obtain differentially expressed genes (DEGs) between HuCCT-1 cells infected with AdGFP or AdHNF4α Overall design: RNA-seq was performed to obtain differentially expressed genes (DEGs) between HuCCT-1 cells infected with AdGFP or AdHNF4α为探究肝细胞核因子4α（HNF4α）在肝内胆管癌（intrahepatic cholangiocarcinoma，ICC）中的潜在分子机制，本研究通过RNA测序（RNA-seq）获取感染AdGFP或AdHNF4α的HuCCT-1细胞之间的差异表达基因（differentially expressed genes，DEGs）。整体实验设计：通过RNA测序（RNA-seq）获取感染AdGFP或AdHNF4α的HuCCT-1细胞之间的差异表达基因（differentially expressed genes，DEGs）