Enhanced Antihyperglycemic Activity of Java Tea (Orthosiphon aristatus)-Based Functional Drink Using Nanoencapsulation

Java Tea-based functional drink has been formulated and reported having bioactive compounds. Nanoencapsulation technology may offer protection toward bioactive compounds as well as enhance the bioavailability. The objective of this study was to investigate the possibility of nanoencapsulation to improve the bioavailability of the Java Tea-based functional drink. Antihyperglycemic activity of mono and double dose nanoencapsulated beverages have been determined in streptozotocin induced diabetic rat, comparing to the ready to drink and microencapsulated beverages. After 2 weeks of oral administration of the three types of beverage, it showed that nanoencapsulated beverage has been able to restore the loss of body weight caused by STZ treatment significantly, and increase the feed intake weekly . Blood glucose level in diabetic rats treated with nanoencapsulated beverage could reduce by 18.68 %, higher than microencapsulated (17.60 %) and ready to drink beverage (5.55 %), respectively. Furthermore, nanoencapsulated beverage treatment has significantly minimized and improved the alteration associated with pancreatic rat diabetes and enhanced the expression of b-cells in the islets of Langerhans. Double dose of nanoencapsulated beverage hasn't given better effect than the single dose. Findings from this study might lend support to the use of Java Tea-based functional drink nanoencapsulated as a novel functional food for the prevention of diabetes.

爪哇茶（Java Tea）基功能饮料已完成配方开发，且被证实含有生物活性化合物。纳米封装技术可对该饮料中的生物活性成分提供保护，同时提升其生物利用度。本研究旨在探究采用纳米封装技术提升爪哇茶基功能饮料生物利用度的可行性。本研究以链脲佐菌素（streptozotocin, STZ）诱导的糖尿病大鼠为模型，测定了单剂量与双剂量纳米封装饮料的抗高血糖活性，并与即饮型饮料及微封装饮料进行对比。经三种饮料连续2周灌胃给药后，结果显示：纳米封装饮料可显著恢复STZ造模导致的大鼠体重下降，并提升大鼠周摄食量。纳米封装饮料给药组糖尿病大鼠的血糖水平可降低18.68%，高于微封装饮料组的17.60%与即饮型饮料组的5.55%。此外，纳米封装饮料给药可显著改善并减轻大鼠糖尿病相关的胰腺病变，并增强胰岛（islets of Langerhans）中β细胞的表达。双剂量纳米封装饮料的给药效果并未优于单剂量组。本研究结果可为将纳米封装爪哇茶基功能饮料作为新型糖尿病预防功能食品提供理论支持。