Phase Separation of Ligand-Activated Enhancers Licenses Cooperative Chromosomal Enhancer Assembly [HiC-seq]. Homo sapiens

Detailed analysis of estrogen mediated enhancer landscape and chromosomal architectural changes specifically in human chromosome 21. Overall design: in situ Hi-C experiments are designed to elucidate 3D chromatin landscape in human breast cancer cells (MCF7) under basal and estrogen treated conditions.

本研究针对人类21号染色体（human chromosome 21）开展雌激素（estrogen）介导的增强子调控图谱与染色体结构变化的精细解析。实验设计概述：本研究通过原位Hi-C（in situ Hi-C）实验，旨在解析人类乳腺癌细胞（MCF7）在基础条件与雌激素处理条件下的三维染色质调控图谱。