Data_Sheet_2_Identification of Candidate Olfactory Genes in the Antennal Transcriptome of the Stink Bug Halyomorpha halys.PDF

The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is a serious agricultural and urban pest that has become an invasive species in many parts of the world. Olfaction plays an indispensable role in regulating insect behaviors, such as host plant location, partners searching, and avoidance of predators. In this study, we sequenced and analyzed the antennal transcriptomes of both male and female adults of H. halys to better understand the olfactory mechanisms in this species. A total of 241 candidate chemosensory genes were identified, including 138 odorant receptors (ORs), 24 ionotropic receptors (IRs), 15 gustatory receptors (GRs), 44 odorant-binding proteins (OBPs), 17 chemosensory proteins (CSPs), and three sensory neuron membrane proteins (SNMPs). The results of semi-quantitative reverse transcription PCR (RT-PCR) assays showed that some HhalOBP and HhalCSP genes have tissue-specific and sex-biased expression patterns. Our results provide an insight into the molecular mechanisms of the olfactory system in H. halys and identify potential novel targets for pest control strategies.

褐纹臭蝽（brown marmorated stink bug，学名*Halyomorpha halys*，隶属于半翅目（Hemiptera）蝽科（Pentatomidae））是一类危害严重的农业与城市害虫，目前已在全球多数区域沦为入侵物种。嗅觉在调控昆虫行为过程中发挥着不可或缺的关键作用，这些行为包括寄主植物定位、配偶搜寻以及天敌规避。本研究对褐纹臭蝽雌雄成虫的触角转录组开展了测序与分析，以期深入解析该物种的嗅觉调控机制。本研究共鉴定得到241个候选化学感受基因，涵盖138个嗅觉受体（odorant receptors, ORs）、24个离子型受体（ionotropic receptors, IRs）、15个味觉受体（gustatory receptors, GRs）、44个气味结合蛋白（odorant-binding proteins, OBPs）、17个化学感受蛋白（chemosensory proteins, CSPs）以及3个感觉神经元膜蛋白（sensory neuron membrane proteins, SNMPs）。半定量逆转录聚合酶链式反应（semi-quantitative reverse transcription PCR, RT-PCR）实验结果表明，部分HhalOBP与HhalCSP基因呈现出组织特异性与性别偏向性的表达特征。本研究结果为解析褐纹臭蝽嗅觉系统的分子调控机制提供了重要视角，同时为开发新型害虫防治策略鉴定了潜在的候选靶点。