Three-color whole cell LLSM imaging data of ER, H2B, and Lyso over 980 time points during mitosis (H2B channel)

This dataset includes the three-color whole cell lattice light-sheet microscopy (LLSM) data of ER, H2B, and Lysosomes over 980 time points at 6 sec intervals in a HeLa cell stably expressing calnexin-mEmerald, H2B-mCherry and Lamp1-Halo during mitosis (only H2B channel here due to the file size restriction, other channels can be found in the same depository with different DOI), which was used to demonstrate SiS-rDL denoising algorithm in our Nature Biotechnology paper (DOI: 10.1038/s41587-022-01471-3). This dataset can be used for non-commercial purposes with proper citations of our NBT paper.本数据集包含内质网（Endoplasmic Reticulum, ER）、组蛋白H2B（H2B）与溶酶体（Lysosomes）的三色全细胞晶格层光显微镜（Lattice Light-Sheet Microscopy, LLSM）成像数据，其成像对象为稳定表达calnexin-mEmerald、H2B-mCherry及Lamp1-Halo的海拉细胞，以6秒为间隔采集了980个时间点的成像数据，完整记录了细胞有丝分裂过程中的动态变化。受文件大小限制，本数据集仅包含H2B通道数据，其余通道数据可通过同一存储库下的不同数字对象标识符（Digital Object Identifier, DOI）获取。本数据集曾用于验证本团队发表于《自然·生物技术》（DOI: 10.1038/s41587-022-01471-3）的SiS-rDL去噪算法，且可在恰当引用该论文的前提下用于非商业用途。