Genome-wide RNAi synthetic lethal screen in Bloom Syndrome cell lines. Homo sapiens

BS cells lackaing both BLM and CDA have developed mechanisms for tolerating endogenous DNA damage and replication stress. With this screen, we aimed to identify genetic interactors enabling BLM- and/or CDA-deficient cells to survive despite constitutive genetic instability.

同时缺失BLM蛋白（BLM protein）与胞苷脱氨酶（cytidine deaminase, CDA）的布卢姆综合征细胞（Bloom syndrome cells, BS cells）已演化出耐受内源性DNA损伤与复制应激的机制。通过本次遗传筛选，我们旨在鉴定能够使BLM缺陷型和/或CDA缺陷型细胞在组成型遗传不稳定状态下仍得以存活的遗传互作因子。