Adipose derived stem cells (ASCs) methylation differences between lean and obese individuals. Adipose derived stem cells (ASCs) methylation differences between lean and obese individuals

Pathological expansion of adipose tissue (AT) in obesity is supported by adipocyte precursors, termed adipose-derived stromal/stem cells (ASCs). Elucidation of mechanisms underlying ASC function may lead to therapeutic interventions to treat fat mass accumulation. Using epigenome-wide association studies, we explored the impact of obesity on the methylation signature of human ASCs. Overall design: Genomic DNA was extracted from cells using the NucleoSpin® Tissue Kit (Macherery-Nagal GmbH). DNA methylation profiles were generated on the Infinium Human-Methylation450K BeadChip (Illumina). The BeadChip was developed to assay more than 480,000 CpG sites and selected CpG loci in parallel. DNA methylation data were processed using GenomeStudio software (Illumina) by applying the default settings.

肥胖状态下脂肪组织（adipose tissue, AT）的病理性扩张，由被命名为脂肪来源基质/干细胞（adipose-derived stromal/stem cells, ASCs）的脂肪细胞前体细胞所介导。阐明脂肪来源基质/干细胞功能的潜在机制，可为治疗体脂过度蓄积提供潜在干预策略。本研究通过表观基因组全关联研究（epigenome-wide association studies），探讨了肥胖对人源脂肪来源基质/干细胞甲基化特征的影响。实验设计概述：采用NucleoSpin®组织提取试剂盒（NucleoSpin® Tissue Kit，Macherery-Nagal GmbH）从细胞中提取基因组DNA。使用Infinium人类甲基化450K微珠芯片（Infinium Human-Methylation450K BeadChip, Illumina）生成DNA甲基化图谱。该微珠芯片可同时检测超过48万个CpG位点及筛选出的CpG基因座。采用GenomeStudio软件（GenomeStudio software, Illumina）以默认参数对DNA甲基化数据进行处理。