Genome wide chromatin changes in shBORIS and shLUC resistant Kelly cells.. Genome wide chromatin changes in shBORIS and shLUC resistant Kelly cells.

We performed ChIPseq on histone modification marks, transcriptional factors and chromatin architectural proteins in both shBORIS and shLUC resistant Kelly cells. Overall design: ChIPseq profiles of shBORIS and shLUC resistant Kelly cells for 4 different marks (BORIS, CTCF, SMC1, H3K27ac) were generated by next generation sequencing, in duplicate, using Illumina NextSeq 500, for a total of 20 individual samples.

我们在shBORIS与shLUC抗性Kelly细胞中，针对组蛋白修饰标记、转录因子及染色质结构蛋白开展了染色质免疫共沉淀测序（ChIPseq）。
总体实验设计：本研究采用Illumina NextSeq 500平台进行下一代测序，构建了shBORIS与shLUC抗性Kelly细胞的4种靶标标记（BORIS、CTCF、SMC1、H3K27ac）的ChIPseq图谱，并设置两次生物学重复，最终共计获得20个独立测序样本。