Vinca minor isolate:Whole plant and transformed roots Transcriptome or Gene expression. Vinca minor isolate:Whole plant and transformed roots

de novo transcriptomic analysis using Illumina Next gen sequencing and miRNAs identification associated with TIAs production, for the first time in whole plant and Agrobacterium mediated hairy roots of Vinca minor. TIAs Pathway gene prediction and associated miRNA were the main targets. SSRs are also predicted in the present study

本研究首次在小蔓长春花（Vinca minor）的全植株及农杆菌介导的毛状根（Agrobacterium-mediated hairy roots）中，采用Illumina二代测序（Illumina Next Generation Sequencing）开展从头转录组分析（de novo transcriptomic analysis），并鉴定与萜类吲哚生物碱（Terpenoid Indole Alkaloids，TIAs）生物合成相关的微RNA（microRNAs，miRNAs）。本研究的核心研究目标为萜类吲哚生物碱通路基因预测及其关联微RNA的挖掘。此外，本研究同时完成了简单序列重复（Simple Sequence Repeats，SSRs）的预测分析。