Expression data from ileum of inducible intestine-specific GATA6 knockout mice. Mus musculus

GATA6 is a transcription factor involved in the differentiation of intestinal epithelial cells into differentiated absorptive epithelial cells. GATA6 is expressed in all segments of the small intestine. We examined the impact of deleting GATA6 from intestinal epithelial cells of the adult ileum Overall design: 6-8 week old Villin-CreERT2 mice or Villin-CreERT2 mice crossed to GATA6 flox/flox mice were treated with a single injection of tamoxifen. (100 mg/ml) for 5 consecutive days. 28 days later the ileum was dissected and RNA prepared from the whole ileal segment using the RNAeasy method with DNAse treatment. Three individual mice were examined in each of the control and GATA6 KO groups.

GATA6是一种参与肠上皮细胞向成熟吸收性上皮细胞分化的转录因子，在小肠各节段均有表达。本研究探究了成年小鼠回肠肠上皮细胞中GATA6敲除的影响。
实验设计：选取6~8周龄的Villin-CreERT2小鼠，以及与GATA6 flox/flox小鼠杂交得到的Villin-CreERT2小鼠，连续5天每日单次注射100mg/ml他莫昔芬。28天后，分离小鼠回肠组织，采用RNAeasy法结合DNA酶处理，从完整回肠节段中提取RNA。对照组与GATA6 KO组各纳入3只独立小鼠样本。