Whole-genome landscape of histone H2AX and ?-H2AX along with related factors in activated cells

Phosphorylation of the histone variant H2AX forms ?-H2AX, which serves as a marker of DNA repair response. Here we provide ChIP-seq-based maps of histone H2AX, ?-H2AX, H2AZ, INO80, SRCAP, and RNA polymerase II in activated T cells. Matched data for H2AX and ?-H2AX in resting T cells and Jurkat cancer T cells are available in GSE25577. Overall design: CD4+ T cells were stimulated in two different ways (IL-2 alone or IL-2 plus anti-CD3 and anti-CD28), and H2AX, ?-H2AX, H2AZ, INO80, and SRCAP profiles were examined by ChIP-seq

组蛋白变体H2AX（histone variant H2AX）经磷酸化后形成γ-H2AX，该产物可作为DNA损伤修复应答的标志物。本研究提供了活化T细胞中组蛋白H2AX、γ-H2AX、组蛋白H2AZ、INO80、SRCAP以及RNA聚合酶II（RNA polymerase II）的染色质免疫共沉淀测序（ChIP-seq）图谱。静息T细胞与Jurkat肿瘤T细胞中H2AX及γ-H2AX的匹配数据可从GSE25577获取。整体实验设计：采用两种不同方式刺激CD4阳性T细胞（仅使用白细胞介素2，或联合使用白细胞介素2、抗CD3抗体与抗CD28抗体），并通过ChIP-seq检测H2AX、γ-H2AX、H2AZ、INO80与SRCAP的结合谱。