Bead-based kinase phosphorylation profiling identifies SRC as a therapeutic target in glioblastoma

Here we describe a bead-based method capable of profiling tyrosine kinase phosphorylations in a multiplexed, high-throughput and low-cost manner. This approach allows for the discovery of tyrosine kinase-activating events, even when the DNA sequence is wild-type. In an effort to pilot the establishment of a tyrosine kinase activation catalog, we profiled tyrosine phosphorylation levels of 62 tyrosine kinases in 130 human cancer lines, and followed-up on the frequent SRC phosphorylation in glioblastoma. Keywords: quantitative measurements of tyrosine phosphorylation levels on tyrosine kinases Total protein lysates were collected from 130 cancer cell lines. Tyrosine phosphorylation levels on 62 tyrosine kinases were measured with the bead assay.

本研究描述了一种基于微珠（bead）的检测方法，可实现多重化、高通量且低成本的酪氨酸激酶（tyrosine kinase）磷酸化谱分析。该方法可用于发现酪氨酸激酶激活事件，即便其DNA序列为野生型（wild-type）。
为试点构建酪氨酸激酶激活图谱，我们对130株人类癌细胞系中62种酪氨酸激酶的酪氨酸磷酸化水平进行了谱分析，并针对胶质母细胞瘤（glioblastoma）中频繁出现的SRC磷酸化展开了后续研究。
关键词：酪氨酸激酶酪氨酸磷酸化水平的定量检测
我们从130株癌细胞系中收集了总蛋白裂解液（total protein lysates），并通过微珠检测法（bead assay）测定了62种酪氨酸激酶的酪氨酸磷酸化水平。